Study on Detecting Colorectal Cancer Micrometastases in Lymph ...

[Abstract]

Objective To develop a nested reverse transcriptase polymerase chain reaction method to amplify Cytokeratin 20mRNA and Matrix Metalloproteinase-7mRNA for detecting colorectal cancer micrometastases in lymph nodes and to evaluate its specificity and sensitivity. Methods The RT-PCR system was set up by using nest primer. The presence of CK20mRNA and MMP-7mRNA was investigated by nested RT-PCR in 100 lymph nodes from 30 colorectal cancer patients who underwent curative operation. Tweenty-five lymph nodes from 10 patients with benign intestinal disease were served as negative control. The results of nested RT-PCR were compared with ones of routine histology. Results The positive rate of CK20mRNA and MMP-7mRNA in 187 lymph nodes from 30 colorectal cancer patients was 40. 11%(75/187), 51. 87%(97/187) respectively, while only 57 samples were histologically positive. The expression of CK20mRNA and MMP-7mRNA in 25 lymph nodes of 10cases of control group were all negative. Conclusion Nested reverse transcriptase ? polymerase chain reaction was a sensitive and specific method to indicate the expression of CK20mRNA and MMP-7mRNA in lymph nodes as a helpful marker of micrometastases of colorectal carcinoma cells. It is superior to routine histopathological assay in examing lymph node metastases. The detection of CK20mRNA and MMP-7mRNA in lymph nodes may contribute to improve the accuracy of clinical staging, determine patients ? prognosis and provide information for adjuvant therapy.

Title: Study on Detecting Colorectal Cancer Micrometastases in Lymph Nodes by Nested Reverse Transcriptase-Polymerase Chain Reaction Amplification of Cytokeratin 20 an

Category: Cancer Cell

Filename: Study on Detecting Colorectal Cancer Micrometastases in Lymph Nodes by Nested Reverse Transcriptase-Polymerase Chain Reaction Amplification of Cytokeratin 20 an.pdf

Pages: 141

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